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Total Count of cells in Stallion Semen
Ejaculates
Directions on how sperm cell density in ejaculates from stallions can be determined by the NucleoCounter SP-100 system is available from ChemoMetec A/S. Together with the NucleoCounter SP-100 these directions are intended to be used at stallion stations and semen collection centers in the production of sperm doses for the artificial insemination of mares. The NucleoCounter SP-100 system determines the sperm cell density in ejaculates with a very high precision. The system makes it possible to perform a precise dilution of the semen giving a more well defined end product, with respect to the total cell count in a dose of sperm.
Semen Doses
Directions on how sperm cell density in stallion semen doses for Artificial Insemination may be determined, by the NucleoCounter SP-100 system, are available from ChemoMetec A/S. Together with the NucleoCounter SP-100 these directions are intended to be used for quality control of semen doses at stallion stations and semen collection centers. The NucleoCounter SP-100 system determines the sperm cell density in semen doses with a very high precision and accuracy. The system can also be used for the determination of the sperm cell density in ejaculates, which have been pre-diluted with extender. Semen doses diluted with the most widely used extenders (also egg yolk containing extenders) can be analyzed with the NucleoCounter SP-100.
Determination of cell viability
The NucleoCounter SP-100 concept offers determination af cell viability in addition to the total count on cells in the sperm sample. Determination of viability is based on the "PI-exclusion" method and the principle is presented here. |
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