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SCC Cassettes
The SCC Cassette is a highly specialised device, which holds the key to several of the unique features of the NucleoCounter SCC-100. The fluorescent dye propidium iodide (PI) is immobilized in the interior flow channels of the disposable cassette. When the cassette has been loaded with approximately 50 µl of sample mixture the PI is dissolved and mixed with the sample, staining the nuclei of the somatic cells. |
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After placement in the NucleoCounter SCC-100 the stained milk sample is automatically transferred to the measurement chamber where the fluorescent image is recorded. The measurement volume is as large as 2 µl.
After analysis the milk sample and the PI is contained within the cassette, which can be safely discarded. This offers a safe handling and disposal of the milk sample and the reagent.
As the SCC Cassette contains the entire flow system as well as the measurement chamber, neither cleaning nor maintenance of the NucleoCounter instrument itself is needed.
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PI
The fluorescent dye propidium iodide (PI) stains the DNA of cell nuclei. The PI intercalates with the DNA with 1 dye molecule per 4-5 DNA base pairs. As little as about 2.8 μg of PI is immobilized in each SCC Cassette.
As PI stains the cell nuclei it needs to be permeable to the dye. The dye cannot penetrate a viable cell and thus it is necessary to lyse the cell membrane prior to staining, when counting the total amount of cells. Reagent C performs an effective lysing of the somatic cells in milk. |
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SCC Cassette |
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