Benefits
Easy operation – as simple as 1-2-3 Very little time is required for training of the operators and no continuous attention is needed
30 sec. analysis time
Calibration free 1. No calibration to cell type as the variation in cell size and DNA content of yeast cells is within the range of the NucleoCounter YC-100 2. No calibration to volume as the volume of sample measured in every single analysis is known, and therefore no calibration to volume is necessary. The thickness of the measurement chamber of each NucleoCassette is measured during production and coded with black dots on each Cassette. The NucleoCounter reads the code. During production the NucleoCounter is calibrated for its viewing area, which will remain unchanged throughout the lifetime of the instrument. The product of the two factors (area multiplied by thickness) gives an accurate sample volume. 3. Durable optical, mechanical and electronical components make the NucleoCounter YC-100 calibration free through out its lifetime No cleaning The NucleoCassette contains the entire flow system (pump, tubings, mixer, reagents and waste container) as well as the measurement chamber. As the NucleoCassette is discarded after use this means neither cleaning nor maintenance of the NucleoCounter YC-100 is needed
Maintenance and service free No maintance as the NucleoCounter YC-100 is made of very durable and stable components. The optics, mechanics and electronics has negligible thermal and aging drift properties. The light source and the linear actuator have been tested to last for >1 x 10E6 samples. No service is needed as no calibration, cleaning nor replacement of components is necessary
Portable/compact The weight of the NucleoCounter YC-100 is 3 kg and the size: 38 x 26 x 22 cm (W x H x D) which makes it easy to move around with.
Safe sample handling and disposal After analysis the sample and the PI is contained inside the cassette, which can be safely discarded.
Excellent reproducibility Results from different NucleoCounter YC-100s can be directly compared: 1.All NucleoCounter YC-100s are calibrated to respond as the same Master NucleoCounter YC-100 at ChemoMetec. 2.The initial calibration will maintain throughout the lifetime of a NucleoCounter YC-100.
Objective cell count Automation eliminates the subjective judgement of the operator. The NucleoCounter YC-100 cell count is therefore an objective and operator-independent count.
Small sample volume Only about 50 μl of sample mixture is loaded into the NucleoCassette. It is possible to perform an accurate and precise estimate of the cell concentration with only a very small sample consumption.
Large analysis volume 1 μl, which is 10-20 times the volume in a haemocytometer, is analysed in one measurement.
Documentation NucleoView software is ideal for data and image processing and for documentation.
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