Benefits
Easy operation - as simple as 1-2-3
Very little time is required for training of operators and no continuous attention is required
30 sec. analysis time
Calibration free
1. No calibration to cell type as the variation in cell size and DNA content of mammalian cells is within the range of the NucleoCounter
2. No calibration to volume as the volume of sample measured in every single analysis is known, and therefore no calibration to volume is necessary. The thickness of the measurement chamber of each NucleoCassette is measured during production and coded with black dots on each Cassette. The NucleoCounter reads the code. During production the NucleoCounter is calibrated for its viewing area, which will remain unchanged throughout the lifetime of the instrument. The product of the two factors (area multiplied by thickness) gives an accurate sample volume.
3. Durable optical components make the NucleoCounter calibration free through out its lifetime
No cleaning
The NucleoCassette contains the entire flow system (pump, tubings, mixer, reagents and waste container) as well as the measurement chamber. As the NucleoCassette is discarded after use no cleaning nor maintenance of the NucleoCounter is needed
Maintenance and service free
No maintance is required, as the NucleoCounter is made of very durable and stable components. The optics, mechanics and electronics has negligible thermal and aging drift properties.
The light source and the linear actuator have been tested to last for >1 x 10E6 samples.
No service is needed as no calibration, cleaning nor replacement of components is necessary
Portable/compact
The weight of the NucleoCounter is 3 kg and the size: 38 x 26 x 22 cm (W x H x D) which makes it easy to move around between laboratories or sites.
Safe sample handling and disposal
After analysis the sample and the PI is contained inside the cassette, which can be safely discarded.
Excellent reproducibility
Results from different NucleoCounters can be directly compared:
1. All NucleoCounters are calibrated to respond as the same Master NucleoCounter at ChemoMetec.
2. The initial calibration will be maintained throughout the lifetime of the NucleoCounter.
Objective cell count
Automation eliminates the subjective judgement of the operator. The NucleoCounter cell count is therefore an objective and operator-independent count.
Small sample volume
Only about 50 μl of sample mixture is loaded into the NucleoCassette. It is possible to perform an accurate and precise estimate of the cell concentration with only a very small sample consumption.
Large analysis volume
2 μl, which is 10-20 times the volume in a haemocytometer, is analysed in one measurement.
Declumping
Cell aggregates are dissolved by lysing cells with Reagent A-100 and B before the total cell count, improving the quality of results.
Documentation
NucleoView software is ideal for additional documentation purposes.
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