NucleoCassette

The NucleoCassette is a highly specialised device, which holds the key to several of the unique features of the NucleoCounter. The fluorescent dye propidium iodide (PI) is immobilized inside the flow channels of the disposable cassette. When the cassette has been loaded with approximately 50 µl of sample mixture the PI is dissolved and mixed with the sample, staining the nuclei of the mammalian cells.

After placement in the NucleoCounter the stained mixture is automatically transferred to the measurement chamber where the fluorescent image is recorded. The measurement volume is as large as 2 µl.
 
After analysis the sample and the PI is contained inside the cassette, which can be safely discarded. This offers a unique and safe sample handling and disposal.
As the NucleoCassette contains the entire flow system as well as the measurement chamber, neither cleaning nor maintenance of the NucleoCounter instrument itself is needed.