What is the precision of the NucleoCounter YC-100?

The precision is described by the Poisson distribution, e.g. counting of random events, where the observed standard deviation is equal to the square root of the number of counted cells. The NucleoCounter YC-100 will analyse about 1µl in each measurement. With the recommended sample preparation for total count (1 part sample and 9 parts Reagent Y100), 1 µl will contain 0.1 µl of the original Sample (10%). If the cell concentration of the sample prior to mixing with reagent is 2 x 10E7 cells/ml, then the analysed volume contains 2000 cells. The square root of 2000 is 48 and thus the relative precision, e.g. Coefficient of Variation (CV), of the result in this case is 2.2% expressed as one standard deviation.