What is the precision of the NucleoCounter SCC-100?

The precision is described by the Poisson distribution, e.g. counting of random events, where the observed standard deviation is equal to the square root of the number of counted cells. The NucleoCounter SCC-100 will analyse about 2µl in each measurement. With the sample preparation (equal parts of sample and Reagent C), 2 µl will contain 1 µl of the original Sample (50%). If the cell concentration of the sample prior to mixing with reagent is 1,000,000 cells/ml, then the analysed volume contains 1,000 cells. The square root of 1,000 is 32 and thus the relative precision, e.g. Coefficient of Variation (CV), of the result in this case is 3.2% expressed as one standard deviation.