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Once the NucleoCassette is loaded is there any risk of liquid spill from the tip?

No. The piston inside the cassette acts as a pump (generating vacuum) when it is pressed during loading. This vacuum causes the sample to flow inside the cassette untill the pressure at both ends of the liquid sample is the same. Therefore the piston acts as a "valve" when there is the same pressure on each side of the liquid sample. The gravity itself can not cause the liquid to move.


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