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What is the purpose of Reagent A-100 and Reagent B?
The lysis Reagent A-100 is used to disrupt of the plasma membranes of the mammalian cells, rendering the nuclei susceptible to staining with propidium iodide (PI). Secondly, the reagent contributes effectively to disaggregation of cell clusters. The stabilizing Reagent B is used after Reagent A-100 to raise the pH value, allowing PI to stain the DNA of the cells more efficiently. |
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