Why is the NucleoCounter calibration free?

1. The NucleoCounter detects fluorescence signals from PI bond to the DNA of cell nuclei. Since the nuclei are uniform in size the variation in the detected PI fluorescence signal is within the factory set detection range, so that no calibration to cell type is necessary.
   


2. The volume of sample measured in every single analysis is known and therefore no calibration to volume is necessary. The thickness of the measurement chamber of each NucleoCassette is measured during production and coded with black dots on each Cassette. The NucleoCounter reads the code.
   During production the NucleoCounter is calibrated for its viewing area, which will remain unchanged throughout the lifetime of the instrument. The product of the two factors (area multiplied by thickness) gives an accurate sample volume.



3. The NucleoCounter optics, mechanics and electronics has negligible thermal and aging drift properties.The instrument therefore maintains its initial factory calibration. The light source and the linear actuator have been tested to last for more than 1 million analysis.