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Mammalian cells can differ with respect to many physical and/or morphological properties. Many methods for the counting of mammalian cells are therefore dependent on a calibration or adjustment in order to count reliable the number of cells present in a sample.
One common property of mammalian cells containing nuclei is the presence of approximately stable amount of DNA. Since the principle used in NucleoCounter for the counting of mammalian cells is based on staining the DNA within the nuclei it becomes evident that the method is reliable in the measurement of various cell species without any adjustment or calibration.
The method has been successfully applied for the counting of heterogeneous cell lines such as adipocytes, CHO cells and hybridomas.
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