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Read more about the applications here.
Yes, we are continuously developing new assays. They will be released as they are perfected.
Yes. The FlexiCyte module enables the user to create user adaptable protocols. Read more here.
At the moment we can analyze mammalian, yeast, insect and fish cells.
No the NC-3000 applications have been validated for a range of different cell types and no calibration is needed in-between different cell lines. In fact no calibration at all is necessary for the user.
All the mammalian cell lines we have tested have been suited for analysis by the NC-3000. This includes but are not limited to; HEK, CHO, MCF-7, NS0 and PER.C6.
Depending on whether you use a cassette, A2 slide or A8 slide the sample consumption is either 60µl, 30µl or 8µl. The analyzed volume is 1µl.
The optimal range is 5x104 – 1x107 cells/ml.
The analysis time is typically between 15-60 seconds.
The NC-3000 is capable of distinguishing between G2 cells and two G1 cells which are in close spatial connection by way of our software algorithm which recognizes the two G1 cells as being two distinct cells and therefore distributes the intensity signal of the double cells evenly.
As with double cells there is no problem working with 1n, 2n ….. Xn cell lines. The software quantifies the intensities and plots its relative to its neighbors and since all the cells are polyploids of a given level the software will have no trouble analyzing the cells.