Go with the flow?
NC-3000™ vs. Flow Cytometry
Fast, easy and flexible
Compared to traditional flow cytometry technology, the NucleoCounter® NC-3000™ has many advantages:
- Get high speed cell count, fixed assays, and user adaptable protocols in one instrument
- Avoid cleaning, rinsing, and clogging
- Don't waste time or money on calibration or service
- Fast start up time - just plug and play
- Get visual inspection of cells
- Small footprint - fits on your desk
- Reduce running costs dramatically
External comparison of NC-3000™ vs. Flow Cytometry
Department of Biology, University of Copenhagen, and BRIC, University of Copenhagen have conducted two comparison studies, comparing the NucleoCounter® NC-3000™ against a flow cytometer: 1) A comparison of DNA quantitation by image and flow cytometry, and 2) Quantitative assesment of apoptotic events using image and flow cytometry.
Conclusion Cell Cycle Analysis
"In this study, we have compared an image cytometer, NucleoCounter NC-3000, and a flow cytometer, BD LSRII, with respect to quantifying DNA content and, thus, determining cell cycle distributions. The obtained data demonstrate that NC-3000 is highly accurate and precise for quantification cellular DNA content when compared with BD LSRII. Thus, we found a high degree of concordance between cell cycle distributions measured by the two cytometric systems. In conclusion, cell cycle distributions quantified by image cytometry is accurate and precise when compared with flow cytometry, with the advantages of cost effectiveness, robustness and potential for morphological confirmation of the measured objects."
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Conclusion Apoptosis Detection
"In this study, we have compared an image cytometer, NucleoCounter NC-3000, and a flow cytometer, BD LSRII, with respect to quantifying different events in the apoptotic process. In the comparison study we have measured phosphatidylserine externalization, collaps of the mitochondrial membrane potential and activation of Caspase 3/7, all of which are well-recognized markers for apoptotic cells. For all three markers NC-3000 was accurate and precise for quantification of apoptotic cells when compared with BD LSRII. Thus, we found a high degree of concordance between the fractions of apoptotic cells measured by the two cytometric systems. In summary, the NC-3000 image cytometer presents a new technology that allows for accurate and quantitative investigations of subtle alterations in cell homeostasis in large populations. In contrast to conventionel flow cytometry NC-3000 enables the user to validate the sample by visual inspection of the individual cells."
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