NC-3000™ Mitochondrial Potential Assay
For easy measurement of changes in the mitochondrial membrane potential
ANALYSIS TIME LESS THAN ONE MINUTE!
Loss of the mitochondrial membrane potential is known to precede apoptosis and chemical-hypoxia-induced necrosis. The lipophilic cationic dye, JC-1, display potential-dependent accumulation in the mitochondria and provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells. In healthy cells, the negative charge established by the intact mitochondrial membrane potential facilitates the accumulation of JC-1 in the mitochondrial matrix. At high concentrations JC-1 forms aggregates and become red fluorescent. In apoptotic cells the mitochondrial potential collapses and JC-1 localizes to the cytosol in its monomeric green fluorescent form.
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Using fluorescence microscopy and image analysis, the NC-3000 system automates detection of cells with collapsed mitochondrial membrane potential. Cells are stained with JC-1 and DAPI. Cellular JC-1 monomers and aggregates are detected as green and red fluorescence, respectively. Mitochondrial depolarization is revealed as a decrease in the red/green fluorescence intensity ratio. Necrotic and late apoptotic cells are detected as blue fluorescent (DAPI) cells.
Key benefits
- Easy discrimination between polarized (healthy) cells, depolarized (apoptotic) cells and necrotic/late apoptotic cells
- Fast automated single cell analysis
- Acquisition and analysis in one simple step
- Analysis time less than one minute
- User friendly protocol with predefined settings
- Standardized results – even with different users
- No calibration required