NC-3000™ DNA Fragmentation Assay
– For easy monitoring of sub-G1 cells
TOTAL ASSAY TIME LESS THAN ONE MINUTE!
During apoptosis, calcium- and magnesium-dependent nucleases are activated which degrade DNA. This means that within the DNA there are nicks and double-strand breaks causing fragmentation. This late event of apoptosis is detected using DNA content analysis to measure cell having less than one DNA equivalent (so-called sub-G1 cells).
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Using fluorescence microscopy and image analysis, the NC-3000 system automates detection of cells with fragmented DNA (sub-G1 cells). After DAPI staining of fixed cells the sample is analyzed using the NC-3000 system and cellular fluorescence is quantified and apoptotic cells with fragmented DNA are seen as a sub-G1 peak in a DNA content histogram displayed on PC screen. Markers in the histogram can be used to demarcate apoptotic cells.
Key benefits
- Easy measurement of DNA fragmentation at the single cell level
- Acquisition and analysis in one simple step
- Analysis time less than one minute
- User friendly protocol with predefined settings
- Standardized results – even with different users
- No RNase treatment required
- No calibration required