NC-3000™ Cell Cycle Assay of Fixed Cells
– For easy measurement of G1/G0, S and G2/M cell cycle phases
ACQUISITION, ANALYSIS AND DATA PRESENTATION IN ONE SIMPLE STEP
The cell cycle represents the most fundamental and important process in eukaryotic cells and is an ordered set of events, culminating in cell growth and division into two daughter cells. In a given population, cells will be distributed among three major phases of the cell cycle: G1/G0 phase (one set of paired chromosomes per cell), S phase (DNA synthesis with variable amount of DNA), and G2/M phase (two sets of paired chromosomes per cell, prior to cell division).
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Using fluorescence microscopy and image analysis, the NC-3000 system automates DNA content quantification and hence, measurements of cell cycle stages. The NC-3000 Cell Cycle Assay uses the nuclear stain, DAPI, to measure DNA content. DAPI binds specifically to double-stranded DNA and, accordingly, there is no requirement for removing RNA prior to DNA content measurements.
This is a prerequisite for other dyes commonly used for measurements of cell cycle phases, such as propidium iodide. After DAPI staining of fixed cells the sample is analyzed using the NC-3000 system and cellular fluorescence is quantified and DNA content histograms are displayed on PC screen. Markers in the displayed histograms can be used to demarcate cells in the different cells cycle stages.
Key Benefits
- Easy measurement of cell cycle phases at the single cell level
- Robust and simple protocol with predefined settings
- Acquisition, analysis and data presentation in one simple step
- Standardized results – even with different users
- No RNase treatment required
- No calibration required